Delivering the Trifecta

Throughput, Speed, and Insights with Multiplexing

The Applied Physiology Laboratory at the University of North Texas (UNT) is a shared resource that provides expertise in immunology, nutrition, and muscle physiology. They often collaborate with partners in the pharmaceutical and nutraceutical industries to provide bioanalytical consultation and biomarker testing services at a more competitive price-point than a contract research organization.

In addition to traditional techniques like western blots and flow cytometry, they use multiplex immunoassays to understand how exercise affects biological outcomes, specifically the impact of inflammation. Multiplexing enables the team to ask and answer research questions they previously couldn’t with traditional ELISAs, according to Jakob Vingren, Co-Director and Associate Professor. With xMAP®-based assays and the FLEXMAP 3D® instrument, they’ve been able to reduce the analysis time by 95% versus singleplex techniques. In addition, they get actionable results faster with less sample input.

Brian McFarlin, Co-Director and Associate Professor, notes the time savings associated with multiplexing is especially critical for industry partners, as the results of one clinical test often determine the course of action for the next. McFarlin prides himself as an early adopter of new technology, and by offering multiplex biomarker assays, the Applied Physiology Laboratory can expedite the research and development process, and better serve their industry partners. View UNT’s video to hear their story.

About the FLEXMAP 3D®

When throughput matters, the FLEXMAP 3D is the multiplexing instrument of choice, able to run up to 144,000 tests per hour in the 96-well format and up to 153,600 test per hour in the 384-well format. Click here to learn more about the FLEXMAP 3D.

How Does xMAP Technology Work?