While a number of PCR based assays may be used for miRNA analysis, few are suitable for the economic analysis of large numbers of miRNAs in one reaction. Those that are capable of multiplexed analysis often lack the single base resolution needed to distinguish a number of miRNAs that differ by only one nucleotide base. The miRNA Chimeric Probe Design Tool will automatically assemble probe sequences in a near ready to order format. Of the 13 tabs in the file, the user only needs to use four of these tabs.
Category Archives - xMAP Multiplexing
扱いが難しいタンパク質をLuminex xMAP® マイクロビーズにカップリングする新しい選択肢
At times, scientists using Luminex® xMAP® Technology may encounter proteins that are difficult to couple to microspheres using traditional EDC-NHS chemistry. There are many reasons why this happens, from protein incompatibility with covalent attachment to sub-optimal conditions during the coupling process. Mix&Go™ Technology for multiplex microspheres helps overcome difficulty in coupling by creating an activated surface that gently yet strongly binds proteins using metal chelation rather than covalent chemistry.
How Do You Know if You’ve Been Vaccinated Against Measles?
Figure from http://www.cdc.gov/measles/cases-outbreaks.html In 2015, over 84 cases (as of January 29th, 2015) …
Designing Your Nucleic Acid Based Assay is Easy!
You do not have to struggle to teach yourself how to design your first Luminex-based nucleic acid assay. We ha…
Don’t Fear Protein Assay Development—Luminex is here!
Are you hiding in the shadows of your laboratory in fear and frustration….wondering how and when you will be s…
10 Ways to Minimize Contamination in a Molecular Laboratory
One of the great advantages of PCR is its exquisite sensitivity. Starting with small fragments of nucleic acid…
Summary: Cytokine Release Syndrome after Blinatumomab Treatment Related to Abnormal Macrophage Activation and Ameliorated with Cytokine-Directed Therapy
We’d like to provide a short summary of Teachey et al., Cytokine Release Syndrome after Blinatumomab Tre…
Mumps Research Reveals that Outbreaks are Still Possible
Before the introduction of widespread vaccination programs, mumps was a very common childhood disease. Althoug…
Getting the Most from Your FLEXMAP 3D Instrument
Did you know that following the regular maintenance schedule for the FLEXMAP 3D® instrument is beneficial for …
Ten Plasma Proteins Possibly Predict Alzheimer’s Disease
The World Alzheimer Report 2009 estimated that 36 million people worldwide are living with dementia, with numb…