On February 28, we honor those fighting rare diseases Patients with a rare disease face many challenges, from …
Category Archives - xMAP Multiplexing
Dogs, Chickens, and Turtles…Oh My! Is Your Pet a Reservoir for Salmonella?
A third of animal acquired illnesses are attributed to Salmonella infection. Pets: an unexpected reservo…
Shooting for the Moon – Proteogenomics – The Next Leap in Precision Cancer Care
APOLLO links proteomic and genomic analysis to develop more effective oncology treatment plans
The Cancer Moonshot is a mission that all of us #CanServe according to Vice President Joe Biden. Announced at the State of the Union address in January 2016, the program aims to double the rate of progress toward a cure for cancer through increased collaboration and investment. Most recently the Applied Proteogenomics Organizational Learning and Outcomes (APOLLO) Network was established, and will combine point-of-care genomic and proteomic analysis in hopes of matching a patient’s tumor types to targeted therapies. A cohort of 8,000 patients undergoing treatment for lung cancer, as part of the Veterans Affairs (VA) and the Department of Defense (DoD) healthcare systems, will be routinely screened at oncologist appointments with the goal of identifying treatment plans that are more effective based on patient’s unique proteogenomic profile.
DigiWest®: Reimaging Western Blotting on the Proteomic Scale
A novel high-throughput protein analysis approach utilizing a bead-based microarray platform
The Western blot has been a staple in the molecular biology tool kit for nearly 40 years. While at Stanford, George Stark was the first to describe the transfer of proteins by capillary action from a gel onto diazobenzyloxymethyl-paper, submitting his work to the Proceedings of the National Academy of Sciences (PNAS) in April 1979.
Have you been to Papua New Guinea? MAGPIX® has.
The first commercially available MAGPIX instrument went to Peter Zimmerman’s field research site to fight malaria
Peter Zimmerman, PhD, Professor of International Health, Biology and Genetics, was the first customer to receive the newly launched MAGPIX® instrument in 2010. While his lab is at Case Western Reserve University (CWRU) in Cleveland, Ohio, the instrument was installed at Zimmerman’s field research site in Papua New Guinea.
PiSCES Reveals More Than Your Horoscope
Novel multiplex matrix network analysis method for the elucidation of protein-protein interaction signatures
While the genome is the molecular recipe, the proteome provides a glimpse into what is happening in real time. Like letters that join together to compose ‘words’, individual proteins can bind to form distinctive protein complexes, where the unique composition of each protein complex signals to the cell a specific message. The human genome was sequenced in 2003, but the different combinations that proteins can form remain largely uncharted due to technological limitations.
Although detecting the subtle changes in the proteome is no easy task, the lab of Adam Schrum was up for the challenge. Schrum and colleagues recently published an article in Science Signaling detailing the development of a new method, PiSCES (Proteins in Shared Complexes Detected by Exposed Surface Epitopes), capable of deciphering the molecular signatures unique to healthy and aberrant signaling networks associated with disease.
PRISM: A Novel Bead-Based Biological Barcode Assay
Enabling large-scale screening of small-molecule libraries in pooled cancer cell lines to discover genotype-specific vulnerabilities
Barcoding is no longer confined to the black stripes of the ubiquitous Universal Product Code (UPC) label found on nearly every product we purchase. Conservationist Paul Hebert was the first to demonstrate the feasibility and utility of DNA barcoding as a way to identify species in 2003.
Applications outside the research lab include the detection of food fraud, where lower quality fish is substituted for a more desirable species. A recent study by OCEANA found that 39 percent of 142 seafood samples collected and DNA tested from grocery stores, restaurants, and sushi venues in New York City were mislabeled.
Partner Product Offerings: Affymetrix | R&D Systems | MilliporeSigma | Luminex
Affymetrix Checkmate on Cancer – get the full view soluble immune checkpoint molecules with the ProcartaPlex® Immuno-Oncology Checkpoint Molecules Panel (14pplex).
R&D Systems, a Bio-Techne Brand Want at custom biomarker assay? Don’t want to build it yourself? The Luminex Assay, by R&D Systems, offers 347 biomarkers to select from enabling you to configure the exact assay you need.
MilliporeSigma Researching Parkinson’s Disease, Alzheimer’s Disease, or Traumatic Brain Injury? The MILLIPLEX® MAP Human Neuroscience Magnetic Bead Panel 1 (product number HNS1MAG-95K) is a new 6-plex kit that requires only 25μL of neat human cerebrospinal fluid (CSF) per assay.
Build It or Buy It: Novel Assays to Identify and Characterize Tuberculosis
Expanding spoligotyping of multidrug-resistant tuberculosis to the Luminex xMAP® platform to increase reproducibility, flexibility and throughput
Approximately one third of the world’s population is infected with the tuberculosis (TB) bacteria, Mycobacterium tuberculosis Complex (MTBC), which is one of the deadliest infectious diseases world-wide. One of the greatest threats to global TB control is the growing prevalence of drug resistant strains. In 2014, multidrug-resistant TB (MDR-TB) accounted for about 3.3% of new TB cases and 20% of previously treated cases. The need for novel molecular methods to rapidly identify TB is critical.
Looking to Reduce Costs, Increase Sample Throughput, and Improve Data Quality? Automate!
Instrumentation solutions for the automation of xMAP® Technology-based multiplex assays
Analyzing a large number of samples or developing a highly multiplexed assay? Consider automating* all or part of your xMAP® workflow.
xMAP Technology provides the flexibility to customize each assay step—for both throughput and multiplexing. Specifically:
- bead coupling
- coupling confirmation
- plate preparation
- incubation
- washing