Multiplexed Gene Expression Profiling with L1000
For our third Webinar Wednesday blog, we’re featuring a talk from John Davis, who is the Profiling Production Lead on the Connectivity Map (CMap) team at the Broad Institute. The CMap links genes, disease states, and cellular perturbations in an easy to use database designed for biologists, chemists, and pharmacologists. The CMap dataset results from L1000 gene expression assay data, which uses xMAP® Technology to measure expression of 978 “landmark” genes.
Visit the CMap website to explore the dataset and use the analysis tool.
In this 20-minute webinar, you’ll learn about:
- The CMap, a functional lookup table for biology, which catalogs 2.2 million profiles representing 28,000 perturbagens and 80 cell lines
- L1000 gene expression assay that uses 384-well plate format and ligation-mediated amplification with the FLEXMAP 3D® for detection of 1000 genes
- Automation solutions for sample loading and detection, which enables up to 264 384-well plates to be run per week resulting in 33,000 profiles per week
- Real-world use cases of CMap: elucidating small-molecule mechanisms, machine learning approaches to recognize compound class signatures, and discovery of novel therapeutics based on genetic loss of function signatures
About Webinar Wednesday
In 2018, we are presenting a new blog series called Webinar Wednesday. Throughout this series, we will share the diverse work of the xMAP multiplexing community by highlighting webinars from the xMAP Connect users group meetings, live webinars, Luminex field application scientists, and Luminex partners. We hope you enjoy the new series!
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